Build a Gel Electrophoresis Chamber
Step- by- step instructions for building gel electrophoresis chamber using inexpensive materials that are easily obtained from local hardware and electronics stores
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TABLE OF CONTENTS
Materials
- Plexiglas® and Tefl on®To fi nd a supplier, look in the Yellow Pages of your local telephone directory under the heading “Plastics - Rods, Tubes, Sheets, Etc.”. Have them cut the pieces of Plexiglas® and Tefl on® to size and the slots in the Gel Carrier Sides (see step 2 under “Construct the Gel Carrier”). The distributor will also be able to provide the Tefl on for the Gel Comb.
- 2 pieces: 3¾˝ by ½˝ by ½˝ thick - for Gel Carrier Sides
- 1 piece: 3¾˝ by 3¾˝ by ¾˝ thick - for Gel Carrier Base
- 2 pieces: 7½˝ by 2½˝ by ¼˝ thick - for Chamber Long Sides
- 2 pieces: 37⁄8˝ by 2½˝ by ¼˝ thick - for Chamber Short Sides
- 2 pieces: 7½˝ by 5˝ by ¼˝ thick - for Chamber Lid and Chamber Bottom
- 1 piece: 7½˝ by 1¼˝ by ¼˝ thick - for Chamber Lid Front
- 6 pieces: ½˝ long pieces of 3⁄8˝ dowel or square rod - for pegs
- Tefl on® - 35⁄8˝ by 1˝ by 1⁄16˝ thick - for Gel Comb
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